Yonsei Advanced Science Institute

Logo and Menu



Kang-Hoon Kim†, Jubeen Yoon†, Christian P. Macks†, Han-Eol Park, Jihyun Youn, Jung-uk Lee, Minji An, Jongseong Park, Jaewon Ko, and Chang Ho Sohn* († equally contributed)
Molecular Decrowding by Tissue Expansion Allows Precise Determination of the Spatial Distribution of Synaptic Proteins at a Nanometer Scale by exTEM
ACS NANO, 17 (11), 9919–9937
Date: May 19, 2023

To understand how the molecular machinery of synapses works, it is essential to determine an inventory of synaptic proteins at a subsynaptic resolution. Nevertheless, synaptic proteins are difficult to localize because of the low expression levels and limited access to immunostaining epitopes. Here, we report on the exTEM (epitope-exposed by expansion-transmission electron microscopy) method that enables the imaging of synaptic proteins in situ. This method combines TEM with nanoscale resolution and expandable tissue-hydrogel hybrids for enhanced immunolabeling with better epitope accessibility via molecular decrowding, allowing successful probing of the distribution of various synapse-organizing proteins. We propose that exTEM can be employed for studying the mechanisms underlying the regulation of synaptic architecture and function by providing nanoscale molecular distribution of synaptic proteins in situ. We also envision that exTEM is widely applicable for investigating protein nanostructures located in densely packed environments by immunostaining of commercially available antibodies at nanometer resolution.

Copyright and Address

  • ADDRESS IBS Hall 50 Yonsei-ro, Seodaemun-gu, Seoul 03722
  • TEL +82-2-2123-4769   FAX +82-2-2123-4606
  • E-MAIL ibs@yonsei.ac.kr
  • Copyright © IBS Center for NanoMedicine,YONSEI UNIV.

Display Page Loading Image